Figure 6. Cohesin proteins in wt and in Stag3^ko/ko testis.

1. Immunoprecipitation of cohesin complexes from wt and Stag3^ko/ko testis nuclear extract using anti-SMC1β antibody (1β) or control IgG as indicated. Precipitates and 5% of input extracts are probed with the indicated antibodies for specific cohesin proteins.
2. Stepwise extraction of proteins from wt and in Stag3^ko/ko testes nuclei using 0, 25, and 250 mM ammonium sulfate (AS) as indicated. Extracts were immunoprobed using the antibodies indicated on the right. *Unspecific band; M = marker; kDa numbers refer to marker signals.

Source data are available online for this figure.