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. 2014 Aug 4;15(9):956–964. doi: 10.15252/embr.201438679

Figure 4. Spliceosome dysfunction compromises sister chromatid cohesion through deregulation of Sororin.

Figure 4

A Precocious sister chromatid separation in HeLa cells transfected with indicated siRNAs [mean ± SD (error bars); N = 3]. At least 100 metaphase spreads were counted. Knockdown efficiency of siRNAs was analyzed by immunoblotting (Supplementary Fig S5A).

B Levels of cohesion factors in HeLa cells transfected with non-targeting (−) or UBL5 siRNAs were determined by immunoblotting.

C HeLa cells transfected with indicated siRNAs were processed for immunoblotting.

D Proportion of RNA-Seq reads from Sororin transcripts mapping to introns in cells transfected with indicated siRNAs [mean ± SEM (error bars); N = 2].

E Density of RNA-Seq reads (pink) mapping to exons (yellow) and introns (blue) in the Sororin (CDCA5) gene, showing retention of intron 1 (marked by red lines).

F Precocious sister chromatid separation in HeLa cells transfected with indicated siRNAs [mean ± SD (error bars); N = 3]. At least 100 metaphase spreads were counted. Knockdown efficiency of siRNAs was analyzed by immunoblotting (Supplementary Fig S5E).

G Sister chromatid cohesion status in metaphase spreads from cells transfected with indicated siRNAs and HA-Sororin cDNA [mean ± SD (error bars); N = 3]. Rescue efficiencies were normalized to the effect of expressing HA-Sororin in siSororin-treated cells. At least 100 metaphase spreads were counted. See also Supplementary Fig S5F.