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. 2014 Aug 5;15(9):965–972. doi: 10.15252/embr.201338386

Figure 1. SUMO regulates Shigella flexneri infectivity in vitro.

Figure 1

A Immunoblot analysis of sumoylation in HeLa cells expressing TAP, TAP-SUMO-1 or TAP-SUMO-2, n = 3.

B Gentamycin assay showing Shigella flexneri entry properties. Values of intracellular bacteria (M90T) are expressed as colony-forming unit and are relative to cells infected with mxiD non-invasive Shigella (n = 3 in three technical replicates). Error bars indicate standard deviation. *P < 0.05; **P < 0.01.

C Immunoblot analysis of HeLa cell lysates using siRNA targeting SAE2.

D Gentamycin assay as in (B) in Hela cells transfected with siRNA against SAE2 and scrambled siRNA as a control (n = 3). Error bars indicate standard deviation. **P < 0.01.

E Analysis of Shigella invasive properties using lysis plaque formation assay in SUMO-expressing HeLa cells. Cells were infected with Shigella flexneri M90T and non-invasive mxiD mutant as a control (n = 4).

F Graphical representation of lysis plaque number. Error bars indicate standard deviation. **P < 0.01.

Source data are available online for this figure.