Sausage stereoview representations of the major family members
of compound (A) AST3-88 (in the H, F, R,W region; RMSD = 1.16 ±
0.45 Å), (B) AMW610, and (C) AMW3-130 (in the H, F, R,W region;
RMSD = 0.66 ± 0.3 Å) aligned on the backbone heavy atoms
of residues 3–7. The His side chain is indicated in green,
the DPhe side chain in yellow, the Arg side chain in blue, and the
Trp side chain in orange. A wide gray backbone indicates greater flexibility
in that domain. Following restrained molecular dynamics (RMD) simulations
for 10 ns, 200 equally spaced structures were energy minimized with
the NMR based restraints. The energy minimized structures were grouped
into conformational families by comparison of the backbone dihedral
angels within the His-Phe-Arg-Trp domain.