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. 2014 Oct 8;9(1):560. doi: 10.1186/1556-276X-9-560

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Copyright © 2014 Yi et al.; licensee Springer.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Intracellular drug delivery of the hybrid PLA NPs/MMC-SPC. Confocal laser scanning microscopy images of H₂₂ cells incubated with the (A,B,C) coumarin-6-PLA NPs/FITC-MMC-SPC and (D,E,F) coumarin-6-PLA NPs/FITC-MMC at the equivalent coumarin-6 concentration for 12 h at 37°C. (A,D) Left column showed the red fluorescence from the cytoskeleton (the cell nuclei were stained with rhodamine-phalloidin). (B,E) Middle column showed the green fluorescence from the drugs (the drugs were labeled with FITC). The merged image of the images in left column and middle column is shown in (E,F) right column. The nuclei were stained with Hoechst 33258 (Sigma-Aldrich, St. Louis, MO, USA) (blue).