Table 3. % Double-Stranded Portion of the Aptamer Sequence, the Melting Temperature of the Hairpin, the Gibbs Free Energy of the Hairpin, and the Gibbs Free Energy of the Duplexed Aptamer Sequence in Each NSET Stem-Loop Beacona.
| % d.s. | Thairpin °C | ΔGhairpin kcal/mol | ΔGduplex kcal/mol | |
|---|---|---|---|---|
| Seq i | 20% | 47.1 | –3.31 | –9.69 |
| Seq ii | 0% | 36.5 | –1.79 | -------- |
| Seq iii | 20% | 40.5 | –2.29 | –8.19 |
| Seq iv | 44% | 50.2 | –4.18 | –24.25 |
| Seq v | 68% | 33.9 | –1.34 | –34.92 |
a
Melting temperatures and ΔG values were calculated with IDT’s oligo analyzer software.