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SE-HPLC profiles of conjugation reaction mixtures (5×, 10×, and 20×) and the representative purified bioconjugate. High molecular weight aggregates were observed with retention times at 11.5–18 min. Molecular weight standards: 670 kDa (14.3 min), 158 kDa (19.2 min), and 1.35 kDa (27.1 min).
