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. 2014 Sep 22;25(10):1801–1810. doi: 10.1021/bc500312w

Scheme 1. Schematic Conjugation of mAb Panitumumab to ICG-sulfo-OSu at Dye:Protein Molar Ratios of 5, 10, or 20.

Scheme 1

SE-HPLC was first applied to purify conjugation products and yield products 1, 2, and 3, respectively. Thereafter, the SE-HPLC purified products were extracted with ethyl acetate to yield products 4, 5, and 6, respectively.