Table 1. Summary Data of ICG-sOSu-Panitumumab (5×, 10×, and 20× Reaction) and ICG-sOSu-HuM195 (10× Reaction) Bioconjugates.
reaction | retention time | aggregates% (before purification) | conjugates% (before purification) | conjugates% (after purification) | yield (%) | # of ICG-sOSu conjugated to panitumumab |
---|---|---|---|---|---|---|
ICG-sOSu-panitumumab | ||||||
5× | 19.2 min | 14 | 52 | 99 | 72 | 1 |
10× | 19.2 min | 30 | 28 | 99 | 53 | 2 |
20× | 19.2 min | 51 | 9 | 99 | 19 | 5 |
ICG-sOSu-HuM195 | ||||||
10× | 18.6 min | 17 | 45 | 99 | 80 | 1 |