Figure 2.

Amino acid sequence alignment of Arabidopsis NRS/ER. A, Comparing the conserved amino acid sequences between NRS/ER and functional bacterial rmlD proteins. Protein sequence alignment was performed using ClustalX (version 1.83; Jeanmougin et al., 1998). E. coli K-12 (GenBank accession no. AAB88399), S. enterica serovar Typhimurium (accession no. 21730455), Mycobacterium tuberculosis H37Rv (accession no. CAB07093), Fusobacterium nucleatum (accession no. NC_003454), and Arabidopsis NRS/ER (accession no. AAR99502). The putative conserved GX₂GX₂G motif and the putative catalytic triad YX₃K (Giraud et al., 2000) are marked. B, Comparison of the conserved amino acid sequences between NRS/ER and three Arabidopsis proteins predicted from the genomic database (At3g14790, At1g53500, and At1g78570). Protein sequences were aligned with ClustalX software.