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Effect of temperature and pH on the relative activity of NRS/ER. A, NRS/ER was incubated with dTDP-6-deoxy-4-keto-Glc and NADPH in reaction buffer for 5 min at different temperatures. B, NRS/ER was incubated for 5 min at 30°C with dTDP-6-deoxy-4-keto-Glc and NADPH in reaction buffer of different 0.1-m sodium phosphate pH values. The data are the average of duplicate assays, and the data are presented as the relative activity of NRS/ER.
