Table II.
Proton chemical shifts and coupling constants of UDP-β-l-Rha synthesized from UDP-α-d-Glc using rmlB and NRS/ER
| Proton | Uracil | Ribose Chemical shifts, δ | UDP-β-l-Rha Rhamnose | Rha coupling constants | |
|---|---|---|---|---|---|
| ppma | Hz | ||||
| 1 | — | 5.96 | 5.21 (5.21) | J1″,P 8.8 | J1″,2″ < 2 |
| 2 | — | 4.36 | 4.08 (4.08) | J2″,3″ 3.4 | |
| 3 | — | 4.28 | 3.62 (3.63) | J3″,4″ 9.3 | |
| 4 | — | 4.34 | 3.35 (3.36) | J4″,5″ 9.8 | |
| 5 | 5.96 | 4.19 | 3.43 (3.43) | J5″,6″ 6.3 | |
| 6 | 7.93 | — | 1.30 (1.30) | ||
a
Chemical shifts are in ppm relative to acetone signal. Rhamnose proton-proton coupling constants in Hz are indicated as well as the 3J1″,P coupling values between phosphate and the H-1 proton of Rha. Values in parentheses are chemical shifts of published data for dTDP-Rha (Nakano et al., 2000); chemical shifts for uridine protons are the same as for UDP-sugars.