Table 2. The List of primers used in Bootscanning analysis.
Primer name | Sequence (5′-3′) | Location | Purpose |
PG3 | AGGGACTTGAAAGCGAAAG | 648-666 | one-step RT-PCR |
RT21 | CTGTATTTCTGCTATTAAGTCTTTTGATGGG | 3539-3509 | one-step RT-PCR |
gag-F2 | ATGGGTGCGAGAGCGTCARTATTAA | 790-814 | Second-round PCR |
RT20 | CTGCCAGTTCTAGCTCTGCTTC | 3462-3441 | Second-round PCR |
POL-1 | TGGAAATGTGGRAARGAAGGAC | 2029-2050 | one-step RT-PCR |
POL-2 | CCTGTCTGYAGRCCCCAATATGTT | 5264-5241 | one-step RT-PCR |
POL-3 | ACTGARAGACAGGCKAATTTTTTAGGGA | 2068-2095 | Second-round PCR |
POL-4 | CTCCTAKTGGGATRTGTACTTCYGARCTTA | 5221-5192 | Second-round PCR |