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. 2014 Oct 15;9(10):e109565. doi: 10.1371/journal.pone.0109565

Figure 4. Protocols and experimental results of rapid immunodot.

Figure 4

(A) Protocols of rapid immunodot for detecting FcεRIα autoantibody in the study. (B) Representative results of rapid immunodot with 12 tested sera from CU patients. Patients 1–6 revealed positive responses and patients 7–12 had negative results against rFcεRIα as compared with the positive control-2 (200 ng of HIgG, designated as (+)Ck-2). Positive control-1 using 800 ng of HIgG was designated as (+)Ck-1 and negative control using normal serum was designated as (-)Ck.