Figure 4. MIP-2 mRNA levels in lung tissues following c-di-GMP treatment. Mice were treated intranasally with c-di-GMP or control c-GMP at 24 hr prior to challenge infection.

MIP-2 mRNA levels in lung were determined prior to challenge and at days 2, 4 and 6 post challenge by real-time PCR. Values shown represent the fold increase of the mean compared to non-infected control mice (five to eight animals per group).