Figure 6.

HPLC separation of PORA- and FCBP-extracted pigments. Isolated etiochloroplasts were fed 5-ALA either in darkness (A and B) or white light (C and D) and stromal polypeptides subjected to immunoprecipitation as described in Figure 4. Pigments were extracted with 100% acetone containing 0.1% diethyl pyrocarbonate. A, HPLC of PORA-extracted pigments on a C18 reverse phase column. B, as A, but showing the same sample diluted with a 10-fold molar excess of synthetic Pchlide b prior to separation. C, HPLC of FCBP-extracted pigments on a C30 reverse phase column. D, as C, but showing the same sample diluted with a 10-fold molar excess of synthetic Chlide b prior to separation. Absorbance spectra were recorded at either 455 nm (A and B) or 435 nm (C and D). Insets show absorbance profiles of natural (A and C) and synthetic (B and D) compounds.