Table 1. Antibodies used for immunoblotting and immunoprecipitation.
Antibody | Species | Source | Cat # | Dilution |
Adaptin α | mouse | BD Transduction Labs | 610501 | 1∶500 |
AIP4/Itch | mouse | BD Transduction Labs | 611199 | 1∶500 |
β-NAP | mouse | BD Transduction Labs | 610892 | 1∶250 |
CIN85 | mouse | Millipore | 05-731 | 1∶500 |
Endophilin 1 | goat | Santa Cruz | sc-10874 | 1∶2000 |
Endophilin 3 | goat | Santa Cruz | sc-8907 | 1∶1000 |
FLAG M2 | mouse | Sigma | F-3165 | 1∶1000 |
Fyn | rabbit | Santa Cruz | sc-16 | 1∶200 |
HA 3F10 | rat | Roche | 11867423001 | 1∶1000; 1∶150 (IP) |
HA.11 | mouse | Covance | MMS-101R | 1∶1000 |
HIP55 | goat | Abcam | ab-2836 | 1∶500 |
His | mouse | Novagen/EMD Millipore | 70796-3 | 1∶1000 |
IgG | rabbit | Jackson Immunoresearch | 011-000-002 | 1 ug per reaction |
Intersectin | rabbit | Regis Kelly (UCSF) | - | 1∶500 |
myc | rabbit | Upstate | 24165 | 1∶1000 |
nArgBP | rabbit | Pietro De Camilli (Yale) | - | 1∶500 |
Nck | mouse | BD Transduction Labs | 610099 | 1∶500 |
Nedd4 | rabbit | Upstate | 07-049 | 1∶5000 |
OSF1 | mouse | Novus Biologicals | H00026578-B02 | 1∶500 |
Ponsin/CAP | rabbit | Upstate | 06-994 | 1∶1000 |
α-spectrin | rabbit | Chemicon | AB992 | 1∶250 |
VGLUT1 | goat | Santa Cruz | sc-23168/sc28940 | 1∶100 |
VGLUT1 | rabbit | Robert Edwards (UCSF) | - | 1∶2000 |
VGLUT1 | Guinea pig | Chemicon | AB-5905 | 1∶5000 |
Vinexin | goat | Santa Cruz | sc-14144 | 1∶200 |
WWP1 | goat | Santa Cruz | sc-11894 | 1∶100 |
WWP2 | goat | Santa Cruz | sc-11897 | 1∶100 |