Table I.

Partial purification of the flavonol 3-OMT from Serratula leaves^a

Purification Step	Total Protein	Specific Activity	Total Activity	Purification	Recovery
	mg	pkat g−1	pkat	-fold	%
(NH4)2SO4	56	0.071	0.004	-	100
Superose-12	20	0.81	0.016	11.5	35.7
Mono-Q	4	3.26	0.013	46	7.2
Adenosine-agarose	0.12	13.8	0.0017	194	0.21

Leaves (approximately 20 g) were extracted with P_i buffer, the homogenate filtered and centrifuged, and the protein that precipitated between 30% and 70% ammonium sulfate saturation was desalted on PD-10 column before successive chromatography on Superose-12, Mono-Q, and adenosine-agarose columns.

^aEnzyme assays were carried out using Q as the substrate, as described in the “Materials and Methods” section.