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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Clin Cancer Res. 2013 Dec 2;20(4):912–925. doi: 10.1158/1078-0432.CCR-13-2281

Figure 3. JQ1 induces G1 arrest and apoptosis in patient-derived MYC-amplified medulloblastoma cell lines.

Figure 3

A Cell cycle analysis of MYC amplified patient medulloblastoma cell lines and one MYCN amplified cell line derived from a GEMM treated with 1µM of JQ1S and JQ1R for 72 hours

B Pooled cell cycle analysis of MYC amplified medulloblastoma cell lines treated with 1µM of JQ1S and JQ1R for 72 hours. Values represent mean ± SD. ANOVA p value<0.001.

C Annexin V/PI apoptosis assays of MYC amplified medulloblastoma cell lines treated with 1µM of JQ1S and JQ1R for 72 hours. Viable cells are negative for annexin V and PI, apoptotic cells are positive for Annexin V and negative for PI and dead cells are positive for both Annexin V and PI.

D, Pooled Annexin V/PI apoptosis analysis of the six MYC-amplified medulloblastoma cell lines following treatment with 1µM of JQ1S and JQ1R for 72 hours. Values represent mean ± SD. Images on right shows induction of the apoptotic protein, BAD, in MB002 cell line, six hours following treatment with 1µM of JQ1S. Scale shown represents 20 micron.