Table I.
Analysis of intercellular fluids from MPAO or PCuAO tobacco plants for transgene expression levels
| MPAO Specific Activity | PCuAO Specific Activity | POD Specific Activity | |
|---|---|---|---|
| units/mg tot. prot. | units/mg tot. prot. | units/mg tot. prot. | |
| F1 | 3.0 | 7.6 × 10−1 | 2.5 × 10−2 |
| F2 | 3.3 | 7.0 × 10−2 | 0.4 × 10−2 |
| I | 0.5 | 5.0 × 10−3 | 1.0 × 10−3 |
| T | 0.8 | 7.0 × 10−3 | 2.0 × 10−3 |
Two successive apoplastic extracts (fractions F1 and F2), intracellular extracts (fraction I), and total soluble extracts (fraction T) were tested for MPAO, PCuAO, and peroxidase (POD) enzymatic activity. The experiment was repeated twice, and data are from a single representative experiment.