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. 2004 Apr;134(4):1414–1426. doi: 10.1104/pp.103.036764

Table III.

ROS-scavenging activity in MPAO or PCuAO transgenic tobacco plants

APX (nmol ASCoxidized min−1 mg−1 prot.) Catalase (nmol H2O2 min−1 mg−1prot.) DHAR (nmol DHAreducedmin−1 mg−1 prot.) Ascorbate Pool ([ASC + DHA]) (nmol g−1 fresh wt) Ascorbate Redox State ([ASC] / [ASC+DHA])
WT 326 ± 27 287 ± 7 191 ± 17 1,470 ± 30 0.87
MPAO 383 ± 17* 295 ± 15 202 ± 22 1,300 ± 110 0.86
PCuAO 401 ± 23* 258 ± 36 269 ± 27* 1,550 ± 80* 0.91*

Ascorbate content and redox enzymes were determined in leaves of 30-d-old wild-type and transgenic plants. The values are the means of four experiments ± sd.

*

Differences from the wild type were significant at P < 0.05 (Student's t paired test).