Table III.
APX (nmol ASCoxidized min−1 mg−1 prot.) | Catalase (nmol H2O2 min−1 mg−1prot.) | DHAR (nmol DHAreducedmin−1 mg−1 prot.) | Ascorbate Pool ([ASC + DHA]) (nmol g−1 fresh wt) | Ascorbate Redox State ([ASC] / [ASC+DHA]) | |
---|---|---|---|---|---|
WT | 326 ± 27 | 287 ± 7 | 191 ± 17 | 1,470 ± 30 | 0.87 |
MPAO | 383 ± 17* | 295 ± 15 | 202 ± 22 | 1,300 ± 110 | 0.86 |
PCuAO | 401 ± 23* | 258 ± 36 | 269 ± 27* | 1,550 ± 80* | 0.91* |
Ascorbate content and redox enzymes were determined in leaves of 30-d-old wild-type and transgenic plants. The values are the means of four experiments ± sd.
Differences from the wild type were significant at P < 0.05 (Student's t paired test).