Table 3. Frequencies of mutations showing escalating trend with the progression of the liver diseases from no liver fibrosis (nLF) or liver fibrosis (LF) to liver cirrhosis (LC) and hepatocellular carcinoma (HCC).
| Position of mutations in HBV genome | non-HCC | HCC n [%] n = 22 | p-value [One way Anova] upto LC | p-value [One way Anova] upto HCC | ||
| Regulatory regions (Nucleotide change) | nLF n[%] n = 17 | LF n[%] n = 9 | LC n[%] n = 20 | |||
| Enhancer I/HBx promoter (1021–1373) | ||||||
| T1050G/A | 3 [17.65] | 2 [22.22] | 7 [35.00] | 12 [54.55] | 0.17 | 0.035 |
| A1053G | 1 [5.88] | 1 [11.11] | 10 [50.00] | 11 50.00] | 0.26 | 0.081 |
| T1050G/A+A1053G | 0 [0.00] | 1 [11.11] | 5 [25.00] | 7 [31.82] | 0.04 | 0.008 |
| Enhancer II (1636–1741) | ||||||
| C1653T | 0 [0.00] | 2 [22.22] | 5 [25.00] | 7 [31.82] | 0.26 | 0.079 |
| BCP (1751–1769) | ||||||
| T1753C | 1[5.88] | 2 [22.22] | 7 [35.00] | 7 [31.82] | 0.12 | 0.14 |
| A1762T/G1764A | 6 [35.29] | 4 [44.44] | 13 [65.00] | 17 [77.27] | 0.13 | 0.01 |
| Precore (1814–1900) | ||||||
| G1862T | 0 [0.00] | 0 [0.00] | 2 [10.00] | 4 [18.18] | 0.33 | 0.05 |
| T1858C | 1 [5.88] | 1 [11.11] | 3 [15.00] | 8 [36.36] | 0.054 | 0.08 |
| G1896A | 0 [0.00] | 0 [0.00] | 0 [0.00] | 6 [27.27] | - | 0.225 |
| ORFs(Amino acid change) | ||||||
| Pre S1 | ||||||
| S98T (T3139A) | 0 [0.00] | 1 [11.11] | 4 [20.00] | 7 [31.82] | 0.041 | 0.001 |
| Surface | ||||||
| T125M (C528T) | 9 [52.94] | 7 [77.78] | 11 [55.00] | 7 [31.82] | 0.952 | 0.408 |
| T127L (C534T) | 0 [0.00] | 0 [0.00] | 4 [20.00] | 9 [40.91] | 0.333 | 0.057 |
| L213I (T791A) | 0 [0.00] | 0 [0.00] | 9 [45.00] | 11 [50.00] | 0.333 | 0.085 |
| HBx | ||||||
| H94Y (C1653T) | 0 [0.00] | 2 [22.22] | 5 [25.00] | 7 [31.82] | 0.269 | 0.079 |
| I127T (T1753C) | 1 [5.88] | 2 [22.22] | 7 [35.00] | 7 [31.82] | 0.129 | 0.106 |
| K130M (A1762T)/V131I (G1764A) | 6 [35.29] | 4 [44.44] | 13 [65.00] | 17 [77.27] | 0.13 | 0.01 |
| Core | ||||||
| T12S (A1934T) | 3 [17.65] | 3 [33.33] | 7 [35.00] | 11 [50.00] | 0.278 | 0.036 |
| S35T (T2003A) | 0 [0.00] | 1 [11.11] | 3 [15.00] | 6 [27.27] | 0.173 | 0.017 |
| T67N (C2100A) | 4 [23.53] | 3 [33.33] | 7 [35.00] | 9 [40.91] | 0.247 | 0.037 |
| E113Q (G2237C) | 0 [0.00] | 3 [33.33] | 5 [25.00] | 8 [36.36] | 0.212 | 0.212 |
| I116L (A2246C) | 3 [17.65] | 4 [44.44] | 8 [40.00] | 11 [50.00] | 0.157 | 0.157 |
| T147C (A2339T/C2340G) | 0 [0.00] | 1 [11.11] | 5 [25.00] | 9 [40.91] | 0.041 | 0.003 |
| P130Q (C2289A) | 0 [0.00] | 0 [0.00] | 3 [15.00] | 7 [31.82] | 0.333 | 0.059 |
| Polymerase | ||||||
| L12V (A2339T/C2340G) | 0 [0.00] | 1 [11.11] | 5 [25.00] | 9 [40.91] | 0.041 | 0.003 |
| F221Y (T791A) | 0 [0.00] | 0 [0.00] | 9 [45.00] | 11 [50.00] | 0.333 | 0.085 |
Significant mutations were indicated in bold (p≤0.05) and marginal significant values were represented in italics.