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. 2014 Oct 15;9(10):e110065. doi: 10.1371/journal.pone.0110065

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© 2014 Harshavardhan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Scheme of BURP-domain containing proteins AtRD22 and AtUSPL1. BURP (named after BNM2, USP, RD22 and Polygalacturonase isozyme) proteins are identified by their C-terminal BURP-domain (red). The BURP domains contain 4 CH-repeats (black). In comparison to AtUSPL1, AtRD22 contains an additional motif, the TXV repeats (yellow), in AtUSPL1 no repetitive domain structure can be found. AGI ID is given in brackets. Position of T-DNA insertions for the used loss-of function mutants is indicated by a green arrow. B. Quantification of AtRD22 and AtUSPL1 mRNA in leaves and roots. The bar diagram indicates the amplification of AtRD22 (left) and AtUSPL1 mRNA relative to ACT2 mRNA (amp. rel. to ACT2 mRNA). Leaf (green) and root (brown) tissue of well watered and drought stressed (2% RWC) plants (N = 5) was analyzed. Asterisks indicate significant differences (T-test: *** p<0.01, ** = p<0.05).