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. 2014 Oct 15;9(10):e110108. doi: 10.1371/journal.pone.0110108

Figure 1. Insulin and TNF-α increases NKG2D ligand expression in 3T3-L1 adipocytes.

Figure 1

(A-C) Differentiated 3T3-L1 cells were treated with insulin (0.5–5 µg/ml) or TNF-α (10–100 ng/ml) for 24 hours. The cells were then analyzed by quantitative PCR to determine the expression levels of (A) Rae-1, (B) Mult-1, and (C) H60b. (D) Differentiated 3T3-L1 cells were treated with insulin, TNF-α, tunicamycin, glucose oxidase, LPS, and palmitate. The cells were then analyzed by quantitative PCR for NKG2D ligand expression. The results were normalized to HPRT levels and are shown as fold-increase over control. The data represent mean ± SD of 3 independent experiments. * p<0.05, ** p<0.01 vs. control.