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. 2004 Apr;134(4):1488–1499. doi: 10.1104/pp.103.038117

Figure 1.

Figure 1.

TSL is more highly expressed in dividing cells. A, Western-blot analysis using α-TSL-peptide affinity-purified antisera of fusion proteins purified from yeast: lane 1, GST-TSL; lane 2, GST. B, Equal amounts of protein extracts from wild-type (lane 1, Wassilewskija ecotype, and lane 3, Columbia ecotype) and tsl (lane 2, tsl-1, and lane 4, tsl-2, in Wassilewskija and Columbia ecotype backgrounds, respectively) flowers were analyzed by western-blot analysis with α-TSL antibody. C, Northern- and western-blot analysis of TSL expression in stationary (st; 10 d after dilution) and exponentially (exp) growing (3 d) Arabidopsis suspension culture cells. Top, Northern-blot analysis of 20 μg total RNA hybridized with TSL cDNA probe (TSL). Hybridization of the same blot after stripping for the presence of 18S rRNA serves as a loading control (middle; 18S). Bottom, TSL protein was detected by western-blot analysis on equal amounts of protein extracts with α-TSL peptide antibody (TSL). Molecular weight markers (numbers) are in kD.