Figure 5.

TKI1 interacts with active forms of TSL. Yeast transformants carrying two-hybrid plasmids expressing fusion proteins (left, TSL-Gal4 DBD fusions; right, TKI1-Gal4 ACT fusions) were lifted and assayed for β-galactosidase activity. The top two panels were stained overnight and the bottom four for 5 h; blue color indicates activity. NQ, TSL N-terminal deletion containing amino acids 73-688; K438E, point mutation changing amino acid 438 from Lys to Glu; ΔN360, TSL N-terminal deletion containing amino acids 360-688; C-TKI1, C terminus of TKI1, amino acids 546-741; TKI1, full-length TKI1.