Table II.
The effect of DPI on the patterns of ROS production in response to ABA or MJ in guard cells of Arabidopsis wild-type and mutant plants
| Plant | Hormone (20 μm)
|
|||||
|---|---|---|---|---|---|---|
| None
|
ABA
|
MJ
|
||||
| No DPI | DPI | No DPI | DPI | No DPI | DPI | |
| Wild type | 100 ± 1.9 | 103 ± 2.5 | 121 ± 2.2 | 104 ± 2.1 | 126 ± 3.5 | 102 ± 3.2 |
| jar1-1 | 96 ± 2.9 | 105 ± 3.2 | 112 ± 3.5 | 106 ± 3.2 | 97 ± 3.4 | 103 ± 3.1 |
| ost1-2 | 99 ± 2.8 | 101 ± 3.0 | 99 ± 3.1 | 98 ± 3.0 | 113 ± 2.8 | 97 ± 3.6 |
| gork1 | 101 ± 3.1 | 102 ± 2.9 | 120 ± 2.7 | 103 ± 3.3 | 127 ± 3.7 | 106 ± 2.9 |
The ROS production was monitored by using fluorescence dye, H2DCF-DA, as described in “Materials and Methods”. The extent of ROS production in guard cells, without 20 μm MJ or 20 μm ABA or 12.5 μm DPI in wild type, was taken as control and 100%. Results are averages (±se) from at least 3 experiments.