Table III.

Reversal by butyrate of stomatal closure by ABA or MJ in guard cells of Arabidopsis wild-type and mutant plants

Plant	Control		ABA (20 μm)		MJ (20 μm)
	No Butyrate	0.5 mm Butyrate	No butyrate	0.5 mm Butyrate	No butyrate	0.5 mm Butyrate
Stomatal aperture (μm)
Wild type	4.12 ± 0.2	4.03 ± 0.5	1.09 ± 0.3	2.72 ± 0.2	0.98 ± 0.1	2.96 ± 0.2
jar1-1	3.41 ± 0.2	3.24 ± 0.7	2.54 ± 0.2	2.75 ± 0.2	3.5 ± 0.3	3.29 ± 0.1
ost1-2	3.87 ± 0.2	3.56 ± 0.4	3.59 ± 0.5	3.61 ± 0.1	1.83 ± 0.1	2.62 ± 0.3
gork1	4.19 ± 0.3	4.07 ± 0.2	2.76 ± 0.2	2.89 ± 0.3	4.22 ± 0.2	4.01 ± 0.4

Modulation of pH change in the presence of butyrate on wild type, jar1-1, ost1-2, and gork1 with respect to stomatal closure in response to 20 μm ABA or MJ. The leaf epidermis were allowed to open in light for 2 h, then ABA or MJ or 0.5 mm butyrate was applied for 2 h. Results are the averages ±se (n = 60) of 3 to 4 independent experiments.