Table III.
Plant
|
Control
|
ABA (20 μm)
|
MJ (20 μm)
|
|||
---|---|---|---|---|---|---|
No Butyrate | 0.5 mm Butyrate | No butyrate | 0.5 mm Butyrate | No butyrate | 0.5 mm Butyrate | |
Stomatal aperture (μm) | ||||||
Wild type | 4.12 ± 0.2 | 4.03 ± 0.5 | 1.09 ± 0.3 | 2.72 ± 0.2 | 0.98 ± 0.1 | 2.96 ± 0.2 |
jar1-1 | 3.41 ± 0.2 | 3.24 ± 0.7 | 2.54 ± 0.2 | 2.75 ± 0.2 | 3.5 ± 0.3 | 3.29 ± 0.1 |
ost1-2 | 3.87 ± 0.2 | 3.56 ± 0.4 | 3.59 ± 0.5 | 3.61 ± 0.1 | 1.83 ± 0.1 | 2.62 ± 0.3 |
gork1 | 4.19 ± 0.3 | 4.07 ± 0.2 | 2.76 ± 0.2 | 2.89 ± 0.3 | 4.22 ± 0.2 | 4.01 ± 0.4 |
Modulation of pH change in the presence of butyrate on wild type, jar1-1, ost1-2, and gork1 with respect to stomatal closure in response to 20 μm ABA or MJ. The leaf epidermis were allowed to open in light for 2 h, then ABA or MJ or 0.5 mm butyrate was applied for 2 h. Results are the averages ±se (n = 60) of 3 to 4 independent experiments.