Table IV.
The effect of butyrate and K252a on alkalization and ROS production by guard cells of Arabidopsis in response to ABA, MJ
| Hormone
|
Rise in pH (fmol H+ GCP−1)
|
ROS Production (% of Control)
|
||||
|---|---|---|---|---|---|---|
| Control | + 0.5 mm Butyrate | + 1 μm K252a | Control | + 0.5 mm Butyrate | + 1 μm K252a | |
| 20 μm | ||||||
| None | 0.34 ± 0.01 | 0.26 ± 0.01 | 0.87 ± 1.2 | 100 ± 3.1 | 101 ± 3.5 | 101 ± 2.7 |
| ABA | 20.8 ± 1.21 | 8.6 ± 1.3 | 0.82 ± 2.1 | 122 ± 3.1 | 110 ± 3.5 | 105 ± 3.1 |
| MJ | 26.5 ± 1.15 | 10.1 ± 1.0 | 0.74 ± 1.4 | 128 ± 2.5 | 114 ± 3.7 | 103 ± 2.9 |
Changes in pH of external solution were recorded after permeation of guard cell plasma membrane by digitonin in control or hormone-treated protoplasts. The ROS production was monitored by using fluorescence dye, H2DCF-DA, as described in “Materials and Methods”. The extent of ROS production in guard cells, without MJ or ABA or butyrate or K252a (control), is taken as 100%. Results are averages (±se) from at least 3 experiments.