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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Medchemcomm. 2014 Jun 27;5(9):1317–1323. doi: 10.1039/C4MD00102H

Figure 4.

Figure 4

(A) Western blot analyses of the heat shock proteins Hsp27, Hsp70 and Hsp90 in MCF-7 breast cancer cells lysis 24 h after treatment with 5f. Concentrations (in μM) of 5f are indicated above each lane. Geldanamycin (G, 500 nM) and DMSO (D) are positive and negative controls. (B) Proteolysis of Hsp90 from TnT reticulocyte lysate incubated under conditions of protein synthesis with vehicle (1% DMSO) 5mM novobiocin and 1mM 5f. An antibody specific to the C-terminus of Hsp90 was used to identify the Hsp90 fragments produced in the presence of increasing amounts of trypsin.