Figure 2.

Characterization of ask2 and ask1 ASK2/ask2. A, Schematic map of the ask1-1 and ask2-1 mutations and primers for identifying genotypes in F2 plants. B, Specificity of the primer combinations (left side of the panel) used in PCR reaction to amplify either ASK1, ask1-1, ASK2, or ask2-1 alleles (right side). C, Relative mRNA expression levels of ASK2 gene in mutants determined by real-time PCR. Black and gray bars represent results from two independent experiments. ASK2 expression in the wild type was considered as 1.