Figure 5.

The interaction between AtTLP9 and ASK1 via yeast two-hybrid assays. p53-SV40 (SV40 T-antigen) and LaminC-SV40 represent known interacting and noninteracting protein partners, respectively. A, Interaction of AtTLP9 and ASK1 with yeast two-hybrid His auxotrophic growth. Yeast cells transformed with the plasmid pairs were cultured on synthetic dropout (SD) medium lacking selected amino acids. SD-W-H, SD media without Trp and His; SD-L-H, SD media without Leu and His; SD-W-L-H, SD media without Trp, Leu, and His. B, β-galactosidase activities of the interaction pairs. Values are the means ± sd of assays from least three independent transformants indicated. β-galactosidase activity is expressed in nmol o-nitrophenol h⁻¹ mg⁻¹ yeast protein.