Figure 7.

AtTLP9 can modulate plant's sensitivity to ABA during seed germination and early seedling development. WT, Columbia wild type; Vector, transgenic plants carrying the empty vector; abi4-1, ABA perception mutant. Plant material was prepared as described in “Materials and Methods.” Each data point represents the mean of triplicate experiments (n = 50 each). The small bars represent se. A, AtTLP9 can modulate the seed germination rate. Seeds of WT, abi4-1, attlp9-1, attlp9-2, and sense transgenic plants S13-2 and S16-1 were plated on ABA-free medium, and germination was scored at time points indicate. B, ABA dose response of seed germination. Seeds were plated on media containing various concentrations of ABA and grown for 2 d. Seedlings with fully emerged radicles were counted. C, Sensitivity of seedling development to ABA. Shown are the percentages of 10-d-old seedlings with developmental arrest and true leaves over total number of seeds planted on Murashige and Skoog media supplemented with 1 μm ABA and 1% Suc.