Figure 8.

Real-time PCR analysis of AtTLP9 expression during seed maturation, germination, and early development. Wild-type (Col-0) seeds were kept in darkness at 4°C for 3 d, and then seeds for germination test were floated on liquid Murashige and Skoog medium and incubated at 22°C under a 16-h-light/8-h-dark photoperiod for 8, 16, 24, 48, and 72 h (0 indicates the time immediately following transfer), while seeds for early development test were plated on solid medium containing 0.7% phytoagar for 5, 8, 10, and 14 d. Poly(A⁺) mRNA was isolated, converted to cDNA, and subjected to real-time PCR analysis as described in “Materials and Methods.” Transcript levels are given as relative values to UBQ10 (the value of 1). Each data point represents the mean of triplicate experiments. The small bars represent se. GS, green siliques; DS, dry seeds.