Table II.
Ch2/Ch1 Normalized (Mean)bc
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Experiment | Channel 1 Description | Channel 2 Description | AtTLP2 | AtTLP7 | AtTLP9 | AtTLP10 |
Hormone Effect | ||||||
Auxin response | msgd seedlings, untreated | msg seedlings; 10 μm indole-3-acetic acid for 30 min | 0.32 | 2.21 | ||
Auxin induction | Mock-treated Col-0 roots | Naphthylacetic acid-treated Col-0 roots | 0.46 | 2.22 | ||
Cytokinin response | Control | 15 min of cytokinin treatment | 2.19 | |||
Abscisic acid insensitive 1 | Wild-type control | Abscisic acid insensitive 1 mutant | 0.49 | 2.6 | 0.35 | |
edr1 mutant | Wild-type leaves | edr1 mutant leaves | 2.33 | |||
Downstream genes of KN1 | Control | Overexpression of KN1-GR in Col-0 background | 0.43 | 2.78 | 4.73 | |
Stress | ||||||
Effects of elevated atmospheric CO2 | Col-0 leaves 360 ppm CO2 | Col-0 leaves 1,000 ppm CO2 | 0.33 | |||
Genes involved in chilling tolerance | Cold-treated Col-0 wild-type tissue | Cold-treated cls8 mutant tissue | 0.22 | 0.15 | ||
Genes involved in potassium nutrition | [K+] = 120 μm | [K+] = 2 mm | 0.34 | 0.2 | ||
Cadmium | Control | 10 μm cadmium-treated plant | 2.72 |
These data are obtained from http://genome-www5.stanford.edu.
All data are corresponding with fluorescence intensities greater than 500 in both channels and ch2:ch1 normalized ratio ≧2.0 or ≦0.5.
When searching dbEST with BLASTN, we find Arabidopsis EST corresponding to four of our AtTLPs represented on microarray data generated by AFGC. AtTLP2 is corresponding to the EST clones 289B10T7 and 173K22T7. AtTLP7, AtTLP9, and AtTLP10 are corresponding to the EST clones 173G1T7, 201E19T7, and F3E6T7, respectively.
It is the mutant of massugu gene, which has meanwhile been officially renamed to NPH4 (nonphototropic hypocotyl 4) gene.