Figure 2.

Analysis of the effect of α-amanitin and cycloheximide on the time courses of seed germination of the tt2-1 Arabidopsis mutant. A, Influence of α-amanitin and cycloheximide on the germination profiles. Seeds were germinated as described in “Materials and Methods,” in the absence (○) or presence of α-amanitin (▴, 1 μm; ⋄, 25 μm; ♦, 50 μm; ▵, 100 μm; •, 250 μm) or 100 μm cycloheximide (♦). The figure shows a representative experiment carried out three times in triplicate. B, Influence of α-amanitin concentration on the kinetic parameters of germination curves for the tt2-1 mutant. T₁₀ (○), T₅₀ (•), and T₉₀ (⋄) values, corresponding to the times (d) required for 10%, 50%, and 90% germination, respectively, were calculated from germination curves as shown in A. C, Influence of α-amanitin concentration on germination frequency monitored 48 h (○), 111 h (•), and 144 h (⋄) after sowing. The figure shows a representative experiment carried out three times in triplicate.