Table II.
Arabidopsis proteins whose abundance was altered by α-amanitin during 1-d imbibition
| Relative Abundancec
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Noa. | Exp. MM | Exp. PI | Arabidopsis Protein Nameb | Cov. | Theo. MM | Theo. PI | AGI No. | H2O/DS | Ama/DS |
| kD | % | kD | |||||||
| 10 | 57.36 | 5.77 | Ribulose bisphosphate carboxylase large chain (precursor) | 28 | 53.47 | 6.25 | AtCg00490 | 0.25 ± 0.08 | 0.86 ± 0.06 |
| 161 | 52.11 | 5.71 | Dihydrolipoamide S-acetyltransferase (mitochondrial) | 10 | 45.07 | 5.93 | At3g25860 | 0.05 ± 0.05 | 0.54 ± 0.05 |
| 194 | 45.50 | 6.15 | 3-Oxoacyl[Acyl-carrier-protein] synthase I | 19 | 50.41 | 8.29 | At5g46290 | 0.46 ± 0.07 | 0.17 ± 0.09 |
| 13 | 73.27 | 6.32 | MLO-like-protein | 15 | 67.23 | 9.55 | At1g61560 | 1.67 ± 0.09 | 1.17 ± 0.08 |
| 32 | 15.16 | 5.75 | β-Subunit of 12S-2 seed storage protein (fragment) | 18 | 20.80 | 7.03 | At1g03880 | 4.31 ± 0.12 | 0.30 ± 0.08 |
| 12 | 16.14 | 5.71 | β-Subunit of 12S-2 seed storage protein (fragment) | 30 | 20.80 | 7.03 | At1g03880 | 3.60 ± 0.14 | 0.24 ± 0.03 |
| 89 | 16.14 | 5.71 | β-Subunit of 12S-2 seed storage protein (fragment) | 36 | 20.80 | 7.03 | At1g03880 | 2.1 ± 0.15 | 0.35 ± 0.05 |
| 69 | 43.67 | 6.29 | 12S seed storage protein precursor | 18 | 50.56 | 6.53 | At1g03880 | 0.16 ± 0.04 | 0.4 ± 0.07 |
DS, tt2-1 dry mature seeds; H2O, tt2-1 seeds incubated for 1 d in water; Ama, α-amanitin, and tt2-1 seeds incubated for 1 d in 500 μm α-amanitin as described in “Materials and Methods”; Cov., coverage; Exp., experimental; Theo., theoretical.
Protein numbering following Gallardo et al. (2001, 2002a) and Arabidopsis seed protein reference maps (http://seed.proteome.free.fr).
Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a).
Data obtained from densitometric analysis of individual spots from 2D gels colored with silver nitrate (see Fig. 5 for an example of 2D gel): normalized spot volume in the tt2-1 mutant seeds incubated for 1 d in water divided by the normalized spot volume in the tt2-1 dry mature seeds (H2O/DS), and in the tt2-1 seeds incubated for 1 d in 500 μm α-amanitin divided by the normalized spot volume in the tt2-1 dry mature seeds (Ama/DS) ±sd, from three different gels and independent extractions.