. 2004 Apr;134(4):1598–1613. doi: 10.1104/pp.103.036293

Table III.

Arabidopsis proteins whose de novo synthesis was inhibited by α-amanitin (data from Figs. 6 and 7)

No.	Exp. MM	Exp. PI	Arabidopsis Protein Name	Cov.	Theo. MM	Theo. PI	AGI No.	Relative Abundance^c
	kD			%	kD			Ama/H₂O
114^a	39.56	5.87	Cytosolic malate dehydrogenase	21	35.57	6.11	At1g04410	0.20 ± 0.01
17^a	72.98	6.57	Phosphoenolpyruvate carboxykinase	17	73.40	6.61	At4g37870	0.16 ± 0.01
168^a	62.91	7.08	Malate synthase	10	63.89	8.02	At5g03860	0.13 ± 0.00
40^a	38.55	6.30	Cytosolic GAPDH	26	36.91	6.62	At3g04120	0.03 ± 0.00
39^a	38.52	6.27	Cytosolic GAPDH	34	36.91	6.62	At3g04120	0.02 ± 0.00
59^b	67.31	5.61	Succinate dehydrogenase	16	69.66	5.86	At2g18450 or At5g66760	≤0.01
103^a	57.95	5.59	Enolase	13	47.72	5.54	At2g36530	≤0.01
121^a	40.92	5.45	Cytosolic phosphoglycerate kinase	16	42.13	5.49	At1g79550	≤0.01
130^b	47.75	5.84	Isocitrate dehydrogenase	16	45.75	6.13	At1g65930	≤0.01
160^a	82.27	5.94	Met synthase	12	84.36	6.09	At5g17920	≤0.01
191^a	61.11	5.81	Mitochondrial processing peptidase	17	59.16	6.30	At3g02090	≤0.01
192^a	60.17	5.88	ATP synthase α-chain (mitochondrial) or expressed protein	14	55.04	6.23	AtMg01190 or At2g07698	≤0.01
201^a	70.29	5.11	Vacuolar ATP synthase catalytic subunit A	28	68.81	5.11	At1g78900	≤0.01
296^b	94.30	5.32	Pyruvate, orthophosphate dikinase	5	98.24	5.55	At4g15530	≤0.01
38^b	57.92	5.62	Leucine aminopeptidase	27	54.51	5.66	At2g24200	≤0.01
128^b	47.45	5.45	Elongation factor 1B-γ	13	46.66	5.36	At1g09640	0.18 ± 0.02
127^a	93.92	5.89	Elongation factor EF-2	31	94.25	5.89	At1g56070	0.07 ± 0.00
105^a	48.63	5.61	Elongation factor 1-γ 2	38	46.40	5.55	At1g57720	≤0.01
156^b	36.04	3.99	Elongation factor 1B α-subunit	15	24.20	4.42	At5g19510	≤0.01
129^a	47.20	5.5	Eukaryotic initiation factor 4A-1	28	46.70	5.47	At3g13920	≤0.01
93^a	42.55	5.27	Actin 7	20	41.73	5.31	At5g09810	0.30 ± 0.02
92^a	53.10	5.59	Myrosinase-binding protein	30	51.21	5.50	At2g33070	0.26 ± 0.02
149^a	64.96	5.18	HSP60	15	61.28	5.66	At3g23990	0.19 ± 0.02
91^a	61.88	5.06	LEA protein-like	13	52.08	5.29	At3g53040	0.02 ± 0.00
13^a	73.27	6.32	MLO-like protein	15	67.23	9.55	At1g61560	≤0.01

H₂O, tt2-1 seeds incubated for 1 d in water and [³⁵S]Met; Ama, α-amanitin, and tt2-1 seeds incubated for 1 d in 500 μm α-amanitin and [³⁵S]Met as described in “Materials and Methods”; Cov., coverage; Exp., experimental; Theo., theoretical.

Listed proteins correspond to previously identified proteins (Gallardo et al., 2001, 2002a).

Listed proteins correspond to proteins identified during this work.

Data obtained from densitometric analysis of individual spots from 2D gels revealed by PhosphorImager analysis (see Fig. 6 for examples of in vivo protein synthesis of seeds labeled with [³⁵S]Met): normalized spot volume in the tt2-1 mutant seeds incubated for 1 d in 500 μm α-amanitin divided by the normalized spot volume in the tt2-1 mutant seeds incubated for 1 d in water ±sd, from three different gels and independent extractions; ≤0.01 means that the accumulation level of the corresponding protein in seeds incubated for 1 d in the presence of 500 μm α-amanitin was close to background.