Figure 2. Cell growth and cell survival of wild-type and autophagy-deficient yeast during nitrogen starvation. (A and B) The number of yeast cells grown in YPD (A) or SD-N (B) was quantified at serial time points. Values represent mean ± SEM of triplicate samples. Similar results were observed in 2 independent experiments. In (A) cell growth curves were fitted to an exponential growth model to determine their doubling time. The difference between growth curves in wild-type (WT) vs. vps30Δ/atg6Δ yeast strains was analyzed using an ANOVA model; statistical results are indicated in each graph. NS, not significant. (C) Wild-type (WT) and vps30Δ/atg6Δ strains were grown in YPD or SD-N for 4 h and tested for survival by plating 100 cells on YPD plates and counting colonies after 48 h at 30 °C. Cell survival was calculated as the percentage of colonies over the total number of plated cells. Bars represent mean ± SEM of triplicate samples. Similar results were observed in 2 independent experiments. NS, not significant; one-way ANOVA for indicated comparison.