Figure 6. BCL2L1 regulates the hypoxia-induced interaction of PGAM5 with FUNDC1. (A) HeLa/6TR- BCL2L1 cells were subjected to hypoxia for 6 h in the presence or absence of Tet. The cell lysates were prepared for immunoprecipitation with an anti-PGAM5 antibody, and the indicated proteins were analyzed the appropriate antibody. (B) BCL2L1 KD or SC cells were subjected to hypoxia for 6 h. The cell lysates were immunoprecipitated with an anti-PGAM5 antibody and immunoblotted with the indicated antibodies. (C) HeLa/6TR- BCL2L1 cells were subjected to hypoxia for 6 h in the presence or absence of Tet. The cell lysates were prepared and incubated with immobilized GST or GST-LC3B beads and detected by western blot analysis. Ponceau S was used to visualize GST and GST-LC3B. (D) BCL2L1 KD or SC cells were transfected with PGAM5 siRNA #1, siRNA #2, or the negative control (NC) for 48 h and followed by hypoxia for 12 h. Samples were analyzed by western blot.