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. 2014 Aug 1;10(10):1787–1800. doi: 10.4161/auto.29989

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graphic file with name auto-10-1787-g3.jpg — Figure 3. Catalytically active PTP4A3 promotes ATG5-dependent LC3 conversion. (A) CHO-Con, CHO-PTP4A1 (PTP4A1), and CHO-PTP4A3 (PTP4A3) cells were cultured in full medium with indicated treatment for the indicated durations before lysis and western blotting analysis with the anti-LC3 and anti-GAPDH antibodies. (B) A2780-Vec, A2780-PTP4A3, and A2780-PDM cells were treated as indicated, and lysed for western blotting analysis with antibodies against LC3 and GAPDH antibodies. (C) A2780-Vec, A2780-PTP4A3, and A2780-PDM cells stably expressing knockdown constructs against nonspecific targets (shScr) or ATG5 (shATG5) were treated as indicated, and lysed for western blotting analysis with the indicated antibodies.