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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Mol Cell. 2014 Jan 30;53(4):672–681. doi: 10.1016/j.molcel.2013.12.029

Figure 7. CP190 binding marks highly RNAPII paused genes thereby favoring long-range interactions.

Figure 7

A. Averaged binding of CP190 in WT cells as quantified by ChIP-Seq (y-axis; in ChIP-Seq reads normalized to IgG control) as a function of high, medium or low (red, green and blue curves) RNA Polymerase II pausing as previously defined (Gilchrist et al., 2010)(see Extended Experimental procedures).

B. Graph showing the percentages of differentially regulated genes in cells expressing mut- compared to WT- Beaf depending on the presence in their promoters of a direct Beaf32 binding site (‘primary targets’), a ChIP-indirect site (‘long-range targets’) with or without a GAF site, as a function of the high, moderate or low RNAPII pausing as previously defined (Gilchrist et al., 2010).

C. Normalized Hi-C profiles by aggregation plots (see Figure 2C for details) probing the enrichment in long-range interactions between direct peaks of Beaf32 (orange triangle/green oval) over promoters harboring high or low RNAPII pausing levels (p-value of 1e-7 and 1, respectively). See also Supplementary Figure S7.

D. Model: CP190-mediated long-range interactions model for the interplay between insulator-regulated gene expression and long-range interactions targeting genes depending on their degree of RNAPII pausing (see text).