Accumulation patterns of ZmHK transcripts in various maize tissues. Total RNA prepared from various maize tissues was subjected to quantitative real-time PCR. A, ZmHK1; B, ZmHK2; C, ZmHK3a. Lb, leaf blade; Ls, leaf sheath; St, stem; Rt, root; Ts, tassel; Hu, husk; Cb, cob; Sk, silk; AL, auricle and ligule; Cc, cultured cells. Accumulation levels of the transcripts are given as the copy number of ZmHK mRNA/0.5 ng total RNA. Real-time PCR was performed in triplicate, and the mean values with sd are shown.