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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Figure 2.

Figure 2

In vivo interaction of NRF-2 with the AMPA receptor subunit gene promoters using the ChIP assay in N2a cells (A) and murine visual cortical tissue (B). Nuclear extract was immunoprecipitated with anti NRF-2α antibodies (NRF-2 IP lane), anti-nerve growth factor receptor p75 antibody (negative control, NGFR IP lane), or no antibody (negative control, No Ab lane). Control reactions for PCR were performed with 0.5% (Input 0.5% IP lane) and 0.1% (Input 0.1% IP lane) of input chromatin. COX6B promoter was used as a positive control, and Exon 8 of NRF-1 was used as a negative control. Results indicate NRF-2 interactions with the tested region on the Gria2 promoter, but not the Gria1, Gria3, or Gria4 promoters, in both N2a cells and murine visual cortical tissue.