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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Figure 3.

Figure 3

Site-directed mutational analysis of promoters using luciferase reporter gene constructs. Wild type promoters (wt) and those with mutated NRF-2 binding site (mut) for COX6b and Gria2 are indicated. COX6b served as a positive control. Mutating the NRF-2 site resulted in a significant decrease in the luciferase activity as compared to the wild type. Similarly, mutating the NRF-2 binding sites on the Gria2 promoter resulted in significant decreases in luciferase activity. KCl depolarization significantly increased promoter activity in all wild type, but not in the COX6b and Gria2 promoters with mutated NRF-2 sites. N = 6 for each construct. ***= P < 0.001; X = NS. All mutants and wild type + KCl are compared to the wild type. All mutant + KCl are compared to mutants.