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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Figure 4.

Figure 4

Effect of RNA interference-mediated silencing of NRF-2α on the expression of COX and AMPA receptor subunit genes. (A) Western blots revealed a down-regulation of NRF-2α and GluA2 protein levels (lane 2) as compared to controls (lane 1), but not GluA1 protein levels, in NRF-2α shRNA-transfected cells. β-actin served as a loading control. N = 3 for each data point; ***= P < 0.001 and *= P < 0.05 when compared to pBS/U6 empty vector controls. (B) As determined by real-time PCR, NRF-2α shRNA transfection in N2a cells down-regulated mRNA levels of NRF-2α and Gria2, but not those of Gria1, Gria3, and Gria4. mRNA levels of the positive control, COX7c, were also reduced with NRF-2α silencing. N = 6 for each data point. ***= P < 0.001 and **= P < 0.01 when compared to pBS/U6 empty vector controls.