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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Figure 5.

Figure 5

Effect of over-expressing NRF-2α and β on the transcript and protein levels of COX7c and AMPA receptor subunit genes. (A) NRF-2α/β over-expression increased protein levels of NRF-2α and NRF-2β, as well as GluA2 (lane 2) as compared to controls (lane 1). Protein levels of GluA1 did not increase significantly with NRF-2α/β over-expression. β-actin served as a loading control. N = 3 for each data point; ***= P < 0.001 when compared to pcDNA3.1 empty vector controls. (B) Real-time PCR revealed an up-regulation of NRF-2α and β mRNA with NRF-2α/β over-expression as compared to pcDNA3.1 empty vector controls. (C) In N2a cells, mRNA levels of Gria2, but not those of Gria1, Gria3, and Gria4, were increased with NRF-2α/β over-expression. mRNA levels of the positive control, COX7c, were also increased with NRF-2α/β over-expression. N = 6 for each data point. **= P < 0.01 and ***= P < 0.001 when compared to pcDNA3.1 empty vector controls.