Figure 6.
Effect of KCl or TTX treatment in the presence of NRF-2 silencing or over-expression, respectively, on the transcript levels of the AMPA receptor subunit genes and COX7c in N2a cells (A) Cells treated for 5 h with 20 mM KCl revealed an up-regulation of all transcripts as compared to pBS/U6 empty vector controls. In the presence of shRNA against NRF-2α, 5 h treatment with 20 mM KCl failed to up-regulate the transcripts of Gria2 and COX7c, but it did up-regulate those of Gria1, Gria3, and Gria4. (B) N2a cells treated for 3 days with 0.4 μM TTX revealed a down-regulation of all tested transcripts as compared to pcDNA3.1 empty vector controls. Over-expression of NRF-2α and β rescued the down-regulation of the COX7c and Gria2 transcripts, but not those of Gria1, Gria3, and Gria4. N = 6 for each data point; ***= P < 0.001 when compared to controls; ### = P < 0.001 and X = non-significant when compared to KCl- or TTX-treated samples.