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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Figure 7.

Figure 7

Effect of NRF-2 silencing and over-expression, with and without KCl or TTX treatment, respectively, on the transcript levels of the AMPA receptor subunit genes in visual cortical neurons. (A) NRF-2α shRNA transfection in primary neurons down-regulated mRNA levels of NRF-2α and Gria2, but not those of Gria1, Gria3, and Gria4. Primary neurons treated for 5 h with 20 mM KCl revealed an up-regulation of all transcripts as compared to pBS/U6 empty vector controls. In the presence of shRNA against NRF-2α, 5 h treatment with 20 mM KCl did not up-regulate transcripts of NRF-2α and Gria2, but it did up-regulate those of Gria1, Gria3, and Gria4. N = 3 for each data point. ***= P < 0.001, **= P < 0.01 and *= P < 0.05 when compared to pBS/U6 empty vector controls. ### = P < 0.001 and X = non-significant when compared to KCl- treated samples. (B) In primary neurons, NRF-2α/β over-expression led to an increase in the transcript levels of NRF2α and Gria2, but not those of Gria1, Gria3, and Gria4. Primary neurons treated for 3 days with 0.4 μM TTX revealed a down-regulation of all tested transcripts as compared to pcDNA3.1 empty vector controls. Over-expression of NRF-2α and β rescued the down-regulation of Gria2 transcripts, but not those of Gria1, Gria3, and Gria4. N = 3 for each data point; ***= P < 0.001 and **= P < 0.01 when compared to controls; ### = P < 0.001 and X = non-significant when compared to TTX-treated samples.